Animal rights activists scuffled with police after an international social media campaign failed to persuade authorities in Spain to spare a dog whose owner contracted ebola - and whose condition has worsened.
Almost 400,000 people signed a petition to save nursing assistant Teresa Romero's dog, Excalibur.
Mrs Romero's condition deteriorated on Thursday and she is now on a ventilator, according to a hospital official.
The 40-year-old is the first person to contract the virus outside of Africa.
Protests erupted outside her apartment in Alcorcon, briefly stopping a police van pulling away with the family pet.
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Gallery: Protests To Save Ebola Victim's Dog
Spanish police block animal rights activists protesting outside the apartment building of the nurse who contracted ebola, Teresa Romero, in Alcorcon, outside Madrid
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Madrid regional authorities said they would euthanise the dog Excalibur to avoid possible contagion, sparking an outcry by animal rights activists to save the pet
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The sign reads: "Zero sacrifice. Ana Mato (Spanish health minister) resign."
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A neighbour tries to get a glimpse of Excalibur as he stands on Ms Romero's balcony. Almost 400,000 people signed a petition to save the dog
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Excalibur barks from the balcony
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A van presumably carrying Excalibur leaves her apartment. Continue through for more pictures
Demonstrators chanted "assassins" as batons were used to clear the road.
Madrid's regional government later said the mixed-breed dog was sedated, euthanised and incinerated.
Authorities had obtained a court order to kill the dog, saying they could not rule out the possibility Excalibur could spread the virus.
On Twitter, the hashtag #SalvemosaExcalibur was tweeted more than 400,000 times in 24 hours.
Mrs Romero's husband, Javier Limon Romero, also made an emotional appeal for the dog to be spared - but the campaign was in vain.
A 2005 study published in a Centres for Disease Control and Prevention journal suggests dogs are susceptible to the virus.
However, there is no documented case of ebola spreading to people from dogs.
A US veterinary health expert criticised the Spanish authorities.
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Gallery: Ebola Crisis: Special Report
"There's never been any evidence of transmission from dogs to humans," said Dr Peter Cowen, a professor at North Carolina State University College of Veterinary Medicine.
"It's never happened in any sense. So we don't have any scientific information that that dog was a risk."
Seven people are currently in isolation at Madrid's Carlos III hospital but Mrs Romero is the only one diagnosed with the virus.
One of the seven, a doctor who treated Mrs Romero over a 16-hour shift, claimed in a letter published by El Pais that he had not been told she had ebola,
:: Watch Ebola Crisis: A Special Report tonight at 8.30pm on Sky News, featuring exclusive footage from Alex Crawford at the heart of the outbreak in Liberia.
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